Compatibility of polyurethane membranes after surface modification with human hypopharyngeal fibroblasts

doi:10.3969/j.issn.2095-4344.2013.34.011

Abstract

Abstract:

BACKGROUND: Polyurethane has good mechanical and physical characteristics and is extensively used in clinical and experimental studies, but its hydrophobicity and histocompatibility are not ideal, which limits its use in tissue engineering as a biomaterial scaffold to some extents.

OBJECTIVE:To observe the hydrophilicity of polyurethane membrane grafted with silk fibroin and glutin and its compatibility with human hypopharyngeal cells.

METHODS:The changes in hydrophilicity of polyurethane membrane grafted with silk fibroin and glutin were detected by contact angle measurements. Human hypopharyngeal fibroblasts were cultured in vitro on polyurethane membrane, silk fibroin-polyurethane membrane, glutin-polyurethane membrane and tissue culture plate. Cell compatibility was compared using cytometry and cell morphology obsevation.

RESULTS AND CONCLUSION: Hydrophilicity of silk fibroin- or glutin-polyurethane membranes significantly increased (P < 0.01). The hydrophilicity of silk fibroin-polyurethane membrane was higher than that of glutin-polyurethane membrane (P < 0.01). The number of cells on the tissue culture plate was the most. The number of human hypopharyngeal fibroblasts on the silk fibroin- or glutin-polyurethane membrane was higher than that on the polyurethane membrane, especially on the silk fibroin-polyurethane membrane. These suggested that hydrophilicity and cell compatibility of silk fibroin- or glutin-polyurethane membrane were elevated.

Key words: biomaterials, material biocompatibility, polyurethane, protein graft, silk fibroin, glutin, human hypopharyngeal fibroblast, tissue engineering

CLC Number:

R318

Kang Cheng, Shen Zhi-sen, Chen Jing-jing, Zhu Ya-bin. Compatibility of polyurethane membranes after surface modification with human hypopharyngeal fibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(34): 6131-6137.

Figures/Tables 6

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